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Octet

Description

The Octet RED96e monitors label-free binding events in real time to calculate on rates (ka), off rates (kd), and affinity constants(KD). Assays are performed using biosensors, reagent and assay kits in 96-well microplates.

The fluidics-free Octet can measure a wide range of sample types; from large biologics to small molecules, purified or in crude form. The Octet RED96e operates with a wide range in temperature (15–40°C). The microplate evaporation cover minimizes sample loss enabling up to 12 hours of unattended run time.

Technical Details

  • Technology: Bio-Layer Interferometry (BLI) based on fiber-optic biosensors
  • Sample volume: 180–220 µL/well, Non-destructive testing, easily recovered
  • Data presentation: Sensorgrams displaying kinetic traces or concentration binding rates, Epitope binning and cross-blocking matrices and trace overlays, Tabulated kinetic or concentration data
  • Automation: Up to 8 assays in parallel; up to 96 assays per 96-well plate
  • Orbital flow capacity: Static or 100–1500 rpm
  • Analysis temperature: Ambient + 4°C to 40°C, in 1°C increments
  • Association rate constant (ka): 101 to 107 M-1 S-1
  • Dissociation rate constant (kd): 10-6 to 10-1 s-1
  • Affinity constant (KD): 1 mM – 10 pM
  • Baseline noise (RMS) <3 pm
  • Baseline drift: < 0.12 nm/hour
  • Quantitation range: 0.5 µg/mL to 2000 µg/mL at 200 rpm, 0.05 µg/mL to 100 µg/mL at 1000 rpm
  • Quantitation precision: CV <10%
  • Sample type: Proteins, antibodies, peptides, serum containing media (up to 25%), DMSO containing buffers, virus-like particles, untreated cell culture supernatants and crude cell lysates
  • Sample plate: Standard, 96-well, black, flat-bottom microplate
  • Molecular weight detection: >150 Da

Applications

  • Protein-protein interactions
  • Antibody pairing, affinity
  • Detection of small molecules

More information can be found on the manufacturer’s website.

Contact Jan Bergervoet

Brand

ForteBio (by Molecular Devices)

Type

RED96e

Organisation

Plant Sciences Group

Department

Bio Interacties en Plantgezondheid

Last edited by Oscar de Vos on 2020-12-29