Description
The Octet RED96e monitors label-free binding events in real time to calculate on rates (ka), off rates (kd), and affinity constants(KD). Assays are performed using biosensors, reagent and assay kits in 96-well microplates.
The fluidics-free Octet can measure a wide range of sample types; from large biologics to small molecules, purified or in crude form. The Octet RED96e operates with a wide range in temperature (15–40°C). The microplate evaporation cover minimizes sample loss enabling up to 12 hours of unattended run time.
Technical Details
- Technology: Bio-Layer Interferometry (BLI) based on fiber-optic biosensors
- Sample volume: 180–220 µL/well, Non-destructive testing, easily recovered
- Data presentation: Sensorgrams displaying kinetic traces or concentration binding rates, Epitope binning and cross-blocking matrices and trace overlays, Tabulated kinetic or concentration data
- Automation: Up to 8 assays in parallel; up to 96 assays per 96-well plate
- Orbital flow capacity: Static or 100–1500 rpm
- Analysis temperature: Ambient + 4°C to 40°C, in 1°C increments
- Association rate constant (ka): 101 to 107 M-1 S-1
- Dissociation rate constant (kd): 10-6 to 10-1 s-1
- Affinity constant (KD): 1 mM – 10 pM
- Baseline noise (RMS) <3 pm
- Baseline drift: < 0.12 nm/hour
- Quantitation range: 0.5 µg/mL to 2000 µg/mL at 200 rpm, 0.05 µg/mL to 100 µg/mL at 1000 rpm
- Quantitation precision: CV <10%
- Sample type: Proteins, antibodies, peptides, serum containing media (up to 25%), DMSO containing buffers, virus-like particles, untreated cell culture supernatants and crude cell lysates
- Sample plate: Standard, 96-well, black, flat-bottom microplate
- Molecular weight detection: >150 Da
Applications
- Protein-protein interactions
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Antibody pairing, affinity
- Detection of small molecules
More information can be found on the manufacturer’s website.